| AAPPS Bulletin
Vol.15 No.4, August 2005 |
Articles
Non-linear Optical Imaging: A Revolution in Biological
Microscopy
(PDF, 144KB)
Chen-Yuan Dong
Department of Physics, National Taiwan University, Taiwan
abstract
In recent years, non-linear optical methods have been
successfully applied for the imaging of biological specimens and
this development revolutionized many areas of life science
research. In this manuscript, I will describe the key features
associated with multiphoton fluorescence and harmonic generation
microscopy. Specifically, the physics of the multiphoton
processes, the unique advantages of multiphoton imaging, key
features of a multiphoton instrumentation, and applications in
oncology, dermatology, and ophthalmology research will be
presented and discussed.
Keywords: multiphoton, harmonic generation, fluorescence,
bioimaging |
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